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| Product Name: |
trypsin in EP/ USP porcine/bovine trypsin
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| Supply Ability: |
1 ton |
| Related proudcts |
porcine trypsin, |
| Specifications |
2500USPu/mg |
| Price Term: |
FOB |
| Port of loading: |
Tianjin |
| Minimum Order |
1kg |
| Unit Price: |
100USD |
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Trypsin
Source: Swine (Bovine) pancreas
General Description
Trypsin is a kind of serine proteolytic enzyme, with molecular weight of 23300 dalton, and is a single peptide chain composed of 223 amino acid residues. With rigorous specificity, the trypsin specifically acts on the peptide linkage constituted by the basic amino acid arginine and leucine. Enzyme easily autolyzes by itself, and its activation also reduces or loses gradually. It is easily soluble in the water, but insoluble in trichloromethane, ethanol, ether and glycerin, and the optimum PH is 8.0~9.0. When the PH is 1.8, it is hardly deactivated when boiled for a short time; if the salt is added into the hot solution, the protein will precipitate, and the enzyme action of filtrate cannot be seen, and Ca2+ plays the role in protecting and activating the trypsin.
The high purity Trypsin of Beijing Wisapple Biotech Co., Ltd is purified by re-crystallization, and then by Ion Exchange Chromatography and ultra-filtration.
Specification
Items Specification Standard
Appearance White or almost white lyophilized powder USP33
Solubility 500,000USP Trypsin Units is soluble in 10ml of water and in
10ml of saline TS. USP33
Loss on drying No more than 5.0% USP33
Residue on Ignition No more than 2.5% USP33
Microbial limits£º
Pseudomonas aeruginosa
Salmonella
Staphylococcus aureus Absent USP33
Chymotrypsin No more than 50 USP units/mg powder USP33
Items Specification Standard
Description white crystalline powder EP6.0
Identification A) A purple color develops within 3 minutes
B) No purple color develops with in 3 minutes EP6.0
Appearance of solution Solution S is not more opalescent than reference suspension ¢ó EP6.0
Absorbance 1) 280nm : 13.5 ~ 16.5
2) 250nm : not more than 7.0 EP6.0
PH 3.0-6.0 EP6.0
Histamine Not more than 1¦Ìg/0.2¦Ìkatal EP6.0
Loss on drying Not more than 5% EP6.0
Limit of chymotrypsin Not more than 1% EP6.0
Microbial limits
Total viable aerobic count
Salmonella
E.Coli
¡Ü10000cfu/g
Absent
Absent EP6.0
Assay |
| Company: |
Beijing Wisapple Biotech Co., Ltd
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| Contact: |
Ms. Wang Lily |
| Address: |
NO.6 Yard,Jinxing West Road,Huangcun, Daxing District, Beijing,China |
| Postcode: |
102600 |
| Tel: |
86 10 84195366-111 |
| Fax: |
86 10 60239806-103 |
| E-mail: |
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